Vacuolar localization of ethylene-induced chitinase in bean leaves.

نویسندگان

  • T Boller
  • U Vögeli
چکیده

The localization of ethylene-induced endochitinase was studied in bean (Phaseolus vulgaris L. cv Saxa) leaves. The specific activity of chitinase in mesophyll protoplasts isolated from the leaves was as high as in tissue homogenates, indicating that most of the enzyme was located intracellularly. Vacuoles isolated and purified from the protoplasts were found to contain most of the intracellular chitinase activity.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Cell Surfaces in Plant-Microorganism Interactions : VI. Elicitors of Ethylene from Colletotrichum lagenarium Trigger Chitinase Activity in Melon Plants.

Treatment of melon leaves or seedlings with elicitors of Colletotrichum lagenarium, a fungal pathogen of melon, increases chitinase activity. In treated leaves, chitinase is enhanced within the first 6 hours and becomes 2 to 10 times higher than in control leaves after 24 hours. Ethylene is increased simultaneously and is correlated with chitinase elicitation. In the presence of aminoethoxyviny...

متن کامل

Preparation and purification of glucanase and chitinase from bean leaves.

Glucanase (endo-beta-1, 3-glucan 3-glucanohydrolase, EC 3.2.1.6, laminarinase, callase) and chitinase (poly-beta-1, 4-[2-acetamido-2-deoxy] -d-glucoside glycanohydrolase, EC 3.2.1.14) were extracted from ethylene-treated bean (Phaseolus vulgaris L. cv. Red Kidney) leaves and purified on hydroxyapatite and carboxymethyl Sephadex columns. The glucanase prepared was homogeneous as judged by analyt...

متن کامل

Analysis of ethylene signal-transduction kinetics associated with seedling-growth response and chitinase induction in wild-type and mutant arabidopsis.

Kinetic aspects of ethylene-mediated signal transduction leading to seedling-growth inhibition and chitinase induction in Arabidopsis were investigated by the introduction of defined mutations in components of these pathways. Dose-response analysis of wild-type responses indicated that the rate-limiting steps for seedling responses and Arabidopsis basic-chitinase induction displayed Michaelis-M...

متن کامل

Functional analysis of DNA sequences responsible for ethylene regulation of a bean chitinase gene in transgenic tobacco.

Expression of at least two genes from bean encoding the defense-related protein chitinase has been shown previously to be transcriptionally regulated by the phytohormone ethylene. We have determined the complete nucleotide sequence of one of these genes, the CH5B gene, which resides on a 4.7-kilobase fragment of bean genomic DNA. The structural gene consists of a single open reading frame and e...

متن کامل

Ethylene Regulation of a Bean Chitinase Gene in Transgenic Tobacco

Expression of at least two genes from bean encoding the defense-related protein chitinase has been shown previously to be transcriptionally regulated by the phytohormone ethylene. We have determined the complete nucleotide sequence of one of these genes, the CH5B gene, which resides on a 4.7-kilobase fragment of bean genomic DNA. The structural gene consists of a single open reading frame and e...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Plant physiology

دوره 74 2  شماره 

صفحات  -

تاریخ انتشار 1984